Library construction is a critical step in high-throughput sequencing, including next-generation sequencing (NGS, such as Illumina platforms) and third-generation sequencing (e.g., PacBio SMRT, Oxford Nanopore). It involves processing DNA or RNA fragments into a library structure compatible with various sequencing platforms. These processing steps include DNA/RNA fragmentation (optional), adapter ligation, amplification, and purification, ultimately generating a sequencing-ready library.
To meet diverse detection needs, iGeneTech has developed multiple library preparation kits that streamline workflows, significantly reduce turnaround time, and are fully compatible with iGeneTech’s automated workstations. This compatibility minimizes human errors, promotes standardized experimentation, and establishes a comprehensive product line of library construction kits.
PART.01 Enzymatic Digestion Library Prep Kit
01 Product Name:
IGT® Enzyme Plus Library Prep Kit V3
02 Product Introduction:
The IGT® Enzyme Plus Library Prep Kit V3 is a universal enzymatic digestion-based DNA library construction kit. Optimized and upgraded from previous versions, this kit minimizes error-prone repair and ligation steps, resulting in a significantly lower Artifact rate. Through a one-step process of DNA fragmentation, end repair, and 3'-dA tailing, followed by adapter ligation, it enables the construction of libraries compatible with sequencing platforms such as Illumina or MGI.
03 Product Advantages:
1. Wide Input Range: Compatible with input amounts ranging from 5ng to 500ng.
2. High Sample Compatibility: Exhibits strong compatibility with diverse sample types.
3. Stable and Efficient Output: Direct enzymatic digestion tolerates up to 0.2 mM EDTA, ensuring consistent library yields.
4. Low Artifact Rate: Reduces artifact formation, enhancing the accuracy of variant detection.
5. Streamlined Workflow: Eliminates the need for sonication, simplifies process, and is fully compatible with automated workstations.
04 Specification & Catalog Number:
PART.02 Fast Library Prep Kit
01 Product Name:
IGT® Fast Library Prep Kit v2.0
02 Product Introduction:
The IGT® Fast Library Prep Kit v2.0 is a universal double-stranded DNA library construction kit suitable for samples such as cell-free DNA (cfDNA) and FFPE DNA. Based on the A-T ligation principle, this kit features a streamlined workflow and is designed for whole-genome and probe-based hybrid capture library construction using 1–500 ng of DNA. By ligating corresponding library adapters, it enables sequencing on high-throughput platforms like Illumina or MGI. Additionally, when paired with molecular barcode technology (UMI), it meets the needs for detecting low-frequency mutations in cfDNA.
03 Product Advantages:
1. Low Input Requirement: Starts with as little as 1ng of sample, offering high library conversion rates and stable, efficient library yields.
2. Broad Sample Compatibility: Works with diverse sample types, including cfDNA, FFPE DNA, and gDNA.
3. Enhanced Mutation Detection: Incorporates molecular barcode technology (UMI) to enable reliable detection of low-frequency variants.
4. Dual-Platform Compatibility: Suitable for both Illumina and MGI sequencing platforms.
04 Specification & Catalog Number:
PART.03 Third-Generation Long-Fragment Library Preparation Kit
01 Product Name:
IGT® Long-Read Tagment DNA Library Prep Kit
02 Product Introduction:
The IGT® Long-Read Tagment DNA Library Prep Kit is a reagent kit for constructing long-fragment DNA libraries using the transposase method. It can be used to build genomic libraries starting with 1 μg of DNA. It offers a long-fragment library construction solution for genomic structural variations (such as deletions, inversions, and translocations) that are difficult to accurately detect in second-generation sequencing, as well as genes with high complexity (pseudogenes, repetitive regions). The constructed libraries can be used for whole-genome library sequencing or target region hybridization capture sequencing in third-generation sequencing.
03 Product Advantages:
1. Simple experimental operation and fast library construction cycle: DNA fragmentation and adapter ligation are completed in one step, and long-fragment library construction can be finished within 4 hours.
2. High library output and stable fragment size.
3. Excellent data indicators: The constructed long-fragment libraries can be used for target region hybridization capture, with excellent capture efficiency and uniformity indicators.
04 Specification & Catalog Number:
PART.04 Strand-Specific RNA Library Preparation Kit
01 Product Name:
IGT® Fast Stranded RNA Library Prep Kit v2.0
02 Product Introduction:
The IGT® Fast Stranded RNA Library Prep Kit v2.0 is a kit for constructing strand-specific libraries from total RNA. It can be used in conjunction with a liquid-phase probe hybridization capture kit for RNA capture sequencing and is suitable for high-throughput sequencing platforms such as Illumina and MGI.
03 Product Advantages:
1. Suitable for library construction with different starting amounts.
2. Highly compatible with different sample types.
3. Simple operation process, no need for sonication, and can be adapted to automated workstations.
04 Specification & Catalog Number:
PART.05 Single-Stranded DNA Library Preparation Kit
01 Product Name:
IGT® ssDNA Library Prep Kit
02 Product Introduction:
The IGT® ssDNA Library Prep Kit is a universal single-stranded DNA library construction kit. It enables the preparation of methylation libraries and genomic libraries starting from 1 ng to 50 ng of DNA. The constructed libraries can be further used for target region hybridization capture sequencing or direct sequencing. During methylation library construction, bisulfite treatment can cause severe DNA damage, fragmentation, and strand separation. Additionally, low-quality or highly degraded DNA often contains abundant single-stranded DNA. The IGT® ssDNA Library Prep Kit enables adapter ligation for single-stranded DNA, significantly improving the utilization of original DNA molecules and enhancing library complexity. This kit demonstrates remarkable advantages in constructing methylation libraries and genomic libraries from low-input samples.
03 Product Advantages:
1. Low-Input Compatibility: Suits low-input samples such as cfDNA and FFPE DNA, with a minimum starting amount of 1ng.
2. Post-BS Strategy: Adopts a Post-BS library construction strategy in methylation library preparation, significantly improving DNA template utilization.
3. Enhanced Library Complexity: Effectively increases library complexity and boosts the yield of valid sequencing data.
4. Versatile Applications: Compatible with whole-genome methylation sequencing, target-region methylation sequencing, whole-genome sequencing, and target-region hybridization capture sequencing.
04 Specification & Catalog Number:
PART.06 Multiplex Amplicon Library Preparation Kit
01 Product Name:
MultipSeq® Library Prep Kit
02 Product Introduction:
The MultipSeq® Library Prep Kit series is designed for multiplex amplicon library construction on Illumina and MGI high-throughput sequencing platforms. It includes multiple versions tailored to different MultipSeq® Primer Pools. Ensure compatibility between the MultipSeq® Library Prep Kit version and the MultipSeq® Primer Pool used before initiating the experiment.
03 Product Advantages:
1. Optimized Workflow: Features a highly optimized experimental system with a starting input as low as 100pg and a high library construction success rate.
2. High Locus Coverage: Achieves high panel locus coverage, reducing the risk and cost of supplementary locus testing using other technologies.
3. Superior Panel Performance: Delivers excellent panel metrics, including high uniformity, which minimizes required sequencing data volume and lowers per-sample sequencing costs.
4. Accurate Detection: Employs a high-fidelity amplification system to ensure precise locus detection accuracy.
04 Specification & Catalog Number:
PART.07 Adapter Kit
01 Product Name:
IGT® Adapter & Primer Series
02 Product Introduction:
IGT® Adapter & Primer is a universal adapter reagent compatible with Illumina and MGI high-throughput sequencing platforms. For the MGI platform, it offers a total of 384 UDI sequences with unique Index numbers. For the Illumina platform, it provides 384 8nt UDI sequences and 768 10nt UDI sequences, meeting the sample pooling requirements for ultra-high data output per lane on high-throughput sequencing platforms. For customers with extremely high sample volume needs, we also offer two specifications of Illumina platform CDI sequences, supporting up to 4,608 samples per lane.
03 Specification & Catalog Number:
PART.08 UMI Adapter Kit
01 Product Name:
IGT® UMI Adapter & Primer Series
02 Product Introduction:
IGT® UMI Adapter & Primer is a universal adapter reagent compatible with Illumina and MGI high-throughput sequencing platforms, incorporating molecular barcode technology (UMI). It offers a total of 384 UDI sequences with unique Index numbers, meeting the sample pooling requirements for ultra-high data output per lane on high-throughput sequencing platforms.
03 Specification & Catalog Number:
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