BisCap® Human CpG Island Panel is targeted for detection of methylation status of 27,000 CpG island and also covers 13 commercial methylation markers. The panel could be widely used in methylation marker screening, early tumor screening, early detection, and etc.
Panel Number | TB018V1hg38 |
Technical Platform | BisCap® Methyl Hybridization Capture Sequencing |
Coverage Size | 21.2 Mb |
Reference Database | RefSeq |
Reference Genome | GRCh38 (hg38) |
Coverage | 27,000 CpG islands and 13 methyl marker regions |
Storage | <-70℃ |
Sequence Platform | Illumina / MGI |
Recommend Sequencing Read Length | PE150 |
Recommend Sequencing Data Size and Depth | 10 Gb / 100X |
Ultra-high coverage that covering 27,000 CpG Islands, 2.03 million CpG sites, as well as 13 commercial methylation markers.
Dual-strain dual-methyl status design for high performance and reduced cost.
IGT® ssDNA Library Prep Kit enables low input amount samples like cfDNA.
Single-base resolution for accurate methyl status detection.
BisCap® Human CpG Island Panel was tested by using NA12878, FFPE DNA, and mock cfDNA (fragmented NA12878 samples) with different input amounts. The results were excellent and consistent among different sample types.
Analysis of methylation levels estimated by BisCap® Human CpG Island Panel and WGBS showed high consistency, indicating BisCap® Human CpG Island Panel is an excellent tool for methylation status detection.
High consistency of methylation levels in repeat experiments indicated that BisCap® Human CpG Island Panel is a stable and reliable panel of methylation status detection.
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