TargetSeq® Cap Beads & Nuclease-Free Water is a reagent kit of streptavidin-coated magnetic beads and nuclease-free water for hybridization capture workflow. The cap beads are covalently coupled with a monolayer of recombinant streptavidin to the surface and further blocked with BSA.

Enzyme Plus Library Prep Kit

Name: Enzyme Plus Library Prep Kit
Brand: iGeneTech Bioscience Co., Ltd.

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Overview

IGT® Enzyme Plus Library Prep Kit V3 provides you a fast and streamlined workflow for NGS library preparation with enzymatic fragmentation strategy. The kit enables tunable fragmentation for different insert sizes according to experimental requirements and is suitable for genomic DNA and FFPE DNA samples with ranged sample input to generate satisfied yield. To achieve good performance with increased sequencing efficiency, IGT® Enzyme Plus Library Prep Kit V3 is designed to have lower artifact rate and could tolerate EDTA residual. Together with IGT® Adapter & indexed primer, the kit is compatible with multiplex sequencing platforms.

Advantages

Suitable for library construction with a wide range of sample input (5~500ng)
Compatible with varies of sample types
Stable and efficient library yield with tolerance to EDTA
Lower artifact rate with higher mutation detection accuracy

Learn more about the AIExome® Human Exome V3-Inherit and AIExome® Human Exome V3-Tumor

Performance

Consistent Performance with Different Sample Input

The IGT® Enzyme Plus Library Prep Kit V3 is compatible with different DNA inputs for library construction. Following the instruction for recommended PCR cycles, you can easily generate library with high yield and stable size.

Tunable Insert Size

The IGT® Enzyme Plus Library Prep Kit V3 is capable to generate desired library inset size by simply varying the enzymatic fragmentation time according to different requirements. The library yield is stable and sufficient for subsequent experiments.

Tolerance to EDTA

The IGT® Enzyme Plus Library Prep Kit V3 could tolerate EDTA with final concentration of 0.2 mM, which will have limited influence on enzymatic fragmentation. For higher EDTA concentration, we provide Enhancer Buffer E v3 for neutralization and you may use it according to the protocol instruction.

Lower Artifact Rate

The IGT® Enzyme Plus Library Prep Kit V3 could have a lower artifact rate compared to other commercial available products, which significantly preserve the sequencing efficiency and accuracy.

Accurate Detection of Mutations

According to the analysis of detected mutations of NA12878 reference standard samples, the results were highly consistent among repetitions of library construction using IGT® Enzyme Plus Library Prep Kit V3, indicating its high accuracy.

Table. Comparison of variation detection results of cell line NA12878 sample among repetitions

	Total Mutations	Consistent Mutations	Accuracy(%)	Sensitivity(%)	Consistency Rate(%)
	Total Mutations	Consistent Mutations	Accuracy(%)	Sensitivity(%)	Consistency Rate(%)
Repeat 1	17980	17743	99.26	99.44	98.70
Repeat 2	17977	17750	99.22	99.45	98.68
Repeat 3	17963	17744	99.29	99.43	98.74

Note: library preparation was performed by using NA12878 reference standard samples with T600V1 probe for target capture. Sequencing was performed on NovaSeq 6000 with PE150. The average sequencing depth for analysis is 139X. By using GATK, the mutations detection consistency were analyzed between the captured targeted regions and database.

Excellent Performance for Target Enrichment

The IGT® Enzyme Plus Library Prep Kit V3 can be used with hybridization-based capture workflow. The tests with TargetSeq® panels of different panel sizes showed excellent performance in capture rate, coverage rate and uniformity.