Selecting Specific PCR Primers with MFEprimer

 Wubin Qu; Chenggang Zhang

 Methods in Molecular Biology 2015;1275:201-213 DOI:10.1007/978-1-4939-2365-6_15

Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind tounintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) how to use the MFEprimer toexamine the specificity of primers.

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