Tn5 transposase can bind insertion sequences in vitro, cut any double-stranded nucleic acid sequence, and then attach the insertion sequences bound by Tn5 to its ends. This characteristic of Tn5 has been widely applied in the construction of whole genome, metagenome, and metatranscriptome libraries[1]. The IGT™ Tagment DNA Library Prep Kit, newly launched by iGeneTech, is a Tn5 transposase library preparation kit that completes DNA fragmentation, end repair, and adapter ligation in one step, making the process simple and only requiring 1.5 hours for complete library construction.
Program Overview
In plant and animal research, targeted capture sequencing technology can be used for molecular marker development, trait screening, strain identification, germplasm relationship analysis, and for population genetics and evolutionary analysis using conserved sequences of species. iGeneTech®️ has independently developed TargetSeq®️ hybrid capture sequencing technology, capable of designing probes for any target region in plant and animal genomics capture sequencing. Paired with the transposase library preparation kit, universal blocking sequences for transposase libraries, and universal Post PCR Primers among other related kits, iGeneTech provides a one-stop solution for plant and animal transposase library preparation and capture sequencing to enterprises, research institutes, and others.
Program Features
Efficient and cost-effective transposase library preparation: The IGT™ Tagment DNA Library Prep Kit is a transposase-based DNA library preparation kit for the Illumina platform, suitable for constructing genomic libraries starting from 50 ng of DNA. The constructed library can be used for whole-genome sequencing or targeted region hybrid capture sequencing. Using in vitro transposition technology, the kit completes DNA fragmentation, end repair, and adapter ligation in just 10 minutes, significantly shortening the library preparation time to less than 1.5 hours while achieving excellent sequencing quality.
Product Advantages
Quick and easy: Complete DNA fragmentation, end repair, and adapter ligation in one step, simplifying the experimental operation.
High throughput: Offers 96 * 12 CDI sequences for customer selection to meet the needs of mixed samples with ultra-high data output in a single lane.
Flexible and efficient: No need for ultrasound fragmentation, can adapt to automated liquid workstations.
Library Preparation Workflow
Figure 1. IGT™ Tagment DNA Library Prep Kit library preparation workflow
Data Performance
Excellent capture efficiency and uniformity indicators in plant and animal Panel hybrid data
Figure 2. Data performance of the IGT™ Tagment DNA Library Prep Kit in plant and animal TargetSeq®️ Panel. Sample input is 50 ng, library preparation with IGT™ Tagment DNA Library Prep Kit, hybridized with 1-Plex (750 ng) and 16-Plex (200 ng per library), selecting a 10k probe plant Panel and a 20k probe animal Panel, sequenced on the NovaSeq 6000 platform PE150.
NXT universal blocking sequences, excellent blocking effect
TargetSeq®️ Eco Universal Blocking Oligo (for Illumina NXT) is a universal blocking sequence for transposase libraries, provided as a one-tube pre-mix for easy operation, supporting multi-hybrid reactions, and effectively reducing costs.
Data Performance
Figure 3. Blocking effect performance of different versions of Universal Blocking Oligo on multi-hybrid reactions. Experiment samples are gDNA, single library input 500 ng, each hybridization reaction input 3 μg (6-Plex), blocked using different versions of Universal Blocking Oligo, probe being TargetSeq®️ NCC Panel (944 kb), sequenced on NovaSeq 6000 or MGISEQ-T7 platform PE150.
Universal Post PCR Primer, compatible with Illumina & MGI platforms, with excellent indicators for QC, insert size, capture efficiency, and uniformity
TargetSeq®️ Universal Post PCR Primer (25 μM) is a universal Post PCR Primer for amplifying captured Illumina libraries, with 5' end phosphorylation modification, and the final library can be directly cyclicized for sequencing on the MGI platform without App-A conversion while also supporting Illumina platform sequencing.
Figure 4. Data performance of TargetSeq®️ Universal Post PCR Primer (25 μM) amplified libraries on Illumina and MGI dual platforms. Experiment samples are gDNA, single library input 750 ng, with different plant and animal Panels for capture, followed by amplification using TargetSeq®️ Universal Post PCR Primer (25 μM) and sequenced respectively on NovaSeq 6000 and MGISEQ-T7 platform PE150.
Leveraging TargetSeq®️ hybrid capture sequencing technology, iGeneTech has developed customized Panels for nearly a hundred different species, accumulating rich project experience. In combination with automated liquid workstations, iGeneTech offers a one-stop solution for plant and animal transposase library preparation and capture sequencing.
Product Information
About iGeneTech®
iGeneTech is a national high-tech enterprise focused on gene capture technology. It has independently developed two gene capture technology platforms with probe hybridization and multiplex PCR, and possesses the capacity for gene capture technology diagnostic services for large sample volumes, aiming to provide globally leading complete solutions for gene capture technology.
Through years of industrial development in gene capture technology, iGeneTech's product line now fully covers the upstream, midstream, and downstream sectors of gene capture, including personalized gene capture product customization and development, gene capture laboratory LIMS systems, fully automated laboratory solutions, data management and interpretation solutions, laboratory quality management systems, NGS sequencing extreme cycle service solutions, ultra-high throughput sample diagnostic service solutions, etc. iGeneTech has become a global gene sequencing technology complete solution provider, offering third-party clinical testing institutions, hospital precision medical centers, gene testing enterprises, scientists, and clinical doctors with all-in-one solutions for the entire cycle of gene testing, highly automated, intelligent, and productized.
CN
