iGeneTech Bioscience Co., Ltd.
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Human Sample ID Panel

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Overview
Performance
Ordering Info

Overview

MultipSeq® Sample ID Research Assay is a simple, cost-efficient and accurate method to identify and track the samples throughout the target sequencing workflow. MultipSeq® Sample ID Research Assay is a small panel with 100 high MAF SNPs, which can also be spiked into customized target sequencing panels including TargetSeq® hybridization capture panels and MultipSeq® multiplex amplicon panels. Through simply comparing the genotyping results of these well selected SNPs, MultipSeq® Sample ID Research Assay can simply help to identify the samples throughout the highly complex NGS workflow and avoid costly re-runs.

Sample-ID-Panel

Product Parameter

Panel Number
A175V1/V2
Technical Platform
MultipSeq® Multiplex Amplicon Sequencing
Number of Primer Pairs
100 pairs
Reference Database
dbSNP、1000Genomes
Reference Genome
GRCh38 (hg38)
Coverage
100 high MAF SNPs in autosomal chromosomes, 2 in X chromosome and 3 in Y chromosome
Storage
-20℃±5℃
Sequence Platform
A175V1:Illumina A175V2:MGI
Recommend Sequencing Read Length
PE150
Recommend Sequencing Data Size and Depth
0.1 Gb / 5000X


Advantages

  • Applicable to various sample types, low input samples, as well as amplification direct from blood, providing an accurate, simple, and economical solution for sample identification and tracking.

  • According to the standard likelihood ratio evaluation, these 100 SNPs are sufficient to achieve a good identification of the whole population.



Learn More About Human Sample ID Panel

Performance

Excellent Performance

Test results of MultipSeq® Sample ID Research Assay using 3 different samples showed that the panel is excellent in data performance, and all SNPs had 100% T 30X coverage rate (3 SNPs on the Y chromosome not detected in the female sample).

sample-id-panel-performance.png


Sample TypeInput Amount (ng)T 30X coverage rate (%)Target mean depth
Promega G304A
40
100
10326.46
Male Saliva Sample
40
100
10792.95
Female Saliva Sample
40
97
9982.89
Consistent Data Performance with Spike-in

Sample ID spike-in module is available and optional in panel customization. Data performance was consistent with or without the spike-in of the sample ID spike-in module in 3 different TargetSeq® panels with different panel sizes.


consistent-data-performance-with-spike-in.png

Consistent Genotyping Results

Genotyping results of AIExome® V2 Plus and MultipSeq® Sample ID Research Assay showed 100% consistent in genotyping results.


consistent-genotyping-results.png

Ordering Info

Sample ID Panel
Product NamePanel NumberSetCat. NoOrder
MultipSeq® Sample ID Research Assay (for Illumina)A175V116 rxnM62191Info
96 rxnM62192Info
960 rxnM62193Info
MultipSeq® Sample ID Research Assay (for MGI DI)A175V216 rxnM62201Info
96 rxnM62202Info
960 rxnM62203Info