TargetSeq® Hema Tumor Fusion RNA Panel is designed for the detection of known and unknown fusion events related to hematological tumors at the RNA level. It includes 141 common fusion genes such as KMT2A, RARα, NUP98, etc. and can detect low abundance transcripts and fusion genes with high sensitivity and accuracy. It is applicable to various scenarios such as disease diagnosis, disease treatment, prognosis determination, and recurrence monitoring.
Panel Number | T508V1RNA |
Technical Platform | TargetSeq® Hybridization Capture Sequencing |
Coverage Size | 851.2 kb |
Reference Database | RefSeq |
Reference Genome | GRCh37 (hg19) |
Coverage | 141 common blood tumor fusion genes |
Storage | <-70℃ |
Sequence Platform | Illumina / MGI |
Recommend Sequencing Read Length | PE150 |
Recommend Sequencing Data Size and Depth | 3 Gb / 2,000X |
Comprehensive detection of hematologic tumor mutations, covering hotspot genes in gonadotropic, myeloid, and partially unknown pedigrees.
Relying on self-developed TargetSeq® hybridization capture technology, the panel is flexible, homogeneous, and capture effective.
100 ng BCR-ABL1 or ETV6-RUNX1 RNA standards (provided by Co-Bioer) was used for library preparation. These libraries were then subjected to capture with TargetSeq® Hema Tumor Fusion RNA Panel followed by sequencing. The resultant data exhibited a high degree of reproducibility.
The BCR-ABL1 and ETV6-RUNX1 fusion gene standards were diluted in a gradient (dilutions of 1-512). RNA libraries were constructed (RNA starting input of 100 ng) and sequenced after hybridization capture using an 851 kb Panel. The results showed that the RNA capture sequencing Panel could still detect two fusion genes at 256-fold dilution. In contrast, BCR-ABL1 and ETV6-RUNX1 fusion genes were not detected in the RNA-Seq data at 4-fold dilution.
| Sequencing Method | Dilution Ratio | BCR-ABL1 Fusion | ETV6-RUNX1 Fusion | ||||
| Input Copy Number | Average Unique Reads | Conclusion | Input Copy Number | Average Unique Reads | Conclusion | ||
| RNA Targeted Sequencing | 1 times | 13400 | 893.0 | Detected | 8800 | 250.5 | Detected |
| 2 times | 6700 | 407.5 | Detected | 4400 | 132.0 | Detected | |
| 4 times | 3350 | 214.0 | Detected | 2200 | 79.5 | Detected | |
| 32 times | 419 | 63.5 | Detected | 275 | 12.5 | Detected | |
| 128 times | 105 | 24.5 | Detected | 68 | 3.5 | Detected | |
| 256 times | 52 | 11.0 | Detected | 34 | 2.5 | Detected | |
| 512 times | 26 | 2.5 | Detected | 17 | 0 | Not detected | |
| RNA-Seq | 4 times | 3350 | 0 | Not detected | 2200 | 0 | Not detected |
| Product Name | Panel Number | Set | Cat. No |
| TargetSeq® Hema Tumor Fusion RNA Panel | T508V1RNA | 16 rxn | PT1006641 |
| 96 rxn | PT1006642 |
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