HLA Panel & MHC Panels Reactive Antibody PRA Screening

Overview

Performance

Ordering Info

Resources

Articles

Overview

TargetSeq® Human HLA Panel and TargetSeq® Human MHC Panel are HLA gene detection products based on TargetSeq® hybridization capture technology developed by iGeneTech. These panels are highly efficient in capturing the polymorphic region and combined with NGS technology to achieve high-precision typing of the HLA gene at 6 sites, and can be widely used in the field of immunology research.

TargetSeq® Human HLA Panel is designed to detect 6 genes related to human leukocyte antigens (HLA) including the full-length sequences of HLA class I molecules A, B, and C genes and the full exon sequences of HLA class II molecules DRB1, DQB1, and DPB1 genes. Based on typing analysis, this panel can be applied to transplant mapping, immune disease research, drug allergy, and other directions.

HLA 15 Genes Panel V2 referenced the Expert Consensus on Standardized Construction of HLA Genotyping Technical Platform and Its Clinical Application. It has targetedly expanded to genotyping detection of 15 genes commonly used in clinical applications. Specifically, probe design covers the full-length sequences of HLA-A, B, and C genes, as well as the full exonic sequences of HLA-DRB1, DQB1, DPB1, DRB3/4/5, DQA1, DPA1, DOA, DOB, DMA, and DMB genes, enabling 6-digit high-resolution accurate genotyping.

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Product Specifications

Product Name	HLA 6 Genes Panel	HLA 15 Genes Panel V2
Technical Platform	TargetSeq® Hybridization Capture Sequencing	TargetSeq® Hybridization Capture Sequencing
Coverage Size	15.7 kb	25.7 kb
Reference Database	IMGT/HLA	IMGT/HLA
Coverage	Full-length sequences of A, B, and C genes, and full exonic sequences of HLA class II molecules DRB1, DQB1, and DPB1	Full-length sequences of HLA-A, B, and C genes, and full exonic sequences of HLA-DRB1, DQB1, DPB1, DRB3/4/5, DQA1, DPA1, DOA, DOB, DMA, and DMB genes
Storage	-20℃±5℃	-20℃±5℃
Sequence Platform	Illumina / MGI	Illumina / MGI
Recommend Sequencing Read Length	PE150	PE150
Recommend Sequencing Data Size and Depth	0.2 Gb / 1,000×	1 Gb / 1,000×

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Advantages

High resolution, enabling 6 sites of high-precision genotyping.
Rigorous design, with probes achieving high coverage of the target region.
Flexible custom, with the possibility of customizing customer-specific HLA typing products based on actual needs.

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Performance

HLA Panel for 6 Sites High-Precision Genotyping

Nearly 100 samples were used for library construction by IGT® Enzyme Plus Library Prep Kit, and TargetSeq® Human HLA Panel was for target enrichment. After sequencing with PE150, HLA-HD analysis software was used for typing. The results showed up to 100% consistancy with the HLA typing results of the conventional PCR SBT method, allowing high-precision genotyping at 6 sites. The figure on the right shows an example of HLA 6 sites high-precision typing results.

Gene	HLA Typing Result
HLA-A	A02:01:01; A01:01:01
HLA-B	B08:01:01; B35:32:02
HLA-C	C07:01:01; C04:01:01
HLA-DQB1	DQB102:01:01G; DQB105:01:01
HLA-DPB1	DPB104:01:01; DPB103:01:01
HLA-DRB1	DRB103:01:01G; DRB107:01:01

Genotyping Validation Results of HLA 15 Genes Panel V2

Multi-gene genotyping validation was conducted using the HLA standard from Nanjing Kebai Biotechnology (CBPQ0007). This Panel can accurately detect the genotyping results of the corresponding standard, with completely consistent genotyping outcomes.

Gene	Cap1	Cap2	Cap3
A	A02:06:01;A31:01:02	A02:06:01;A31:01:02	A02:06:01;A31:01:02
B	B40:02:01;B46:01:01	B46:01:01;B40:02:01	B40:02:01;B46:01:01
C	C01:02:01;C03:04:01	C01:02:01;C03:04:01	C01:02:01;C03:04:01
DRB1	DRB104:07:01;DRB114:03:01	DRB104:07:01;DRB114:03:01	DRB114:03:01;DRB104:07:01
DRB3	DRB301:01:02;DRB301:01:02	DRB301:01:02;DRB301:01:02	DRB301:01:02;DRB301:01:02
DRB4	DRB401:03:01;DRB401:03:01	DRB401:03:01;DRB401:03:01	DRB401:03:01;DRB401:03:01
DQB1	DQB103:01:01;DQB103:02:01	DQB103:01:01;DQB103:02:01	DQB103:01:01;DQB103:02:01
DQA1	DQA105:03:01;DQA103:01:01	DQA105:03:01;DQA103:01:01	DQA105:03:01;DQA103:01:01
DPB1	DPB105:01:01;DPB102:01:02	DPB105:01:01;DPB102:01:02	DPB105:01:01;DPB102:01:02
DPA1	DPA101:03:01;DPA102:02:02	DPA102:02:02;DPA101:03:01	DPA101:03:01;DPA102:02:02
DOA	DOA01:01:02;DOA01:01:01	DOA01:01:02;DOA01:01:01	DOA01:01:02;DOA01:01:01
DOB	DOB01:01:05;DOB01:04:01	DOB01:04:01;DOB01:01:05	DOB01:04:01;DOB01:01:05
DMA	DMA01:01:01;DMA01:01:01	DMA01:01:01;DMA01:01:01	DMA01:01:01;DMA01:01:01
DMB	DMB01:03:01;DMB01:02:01	DMB01:03:01;DMB01:02:01	DMB01:02:01;DMB01:03:01

Note: This sample does not include genetic information of DRB5.

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Ordering Info

HLA Panel & MHC Panel

Product Name	Panel Number	Set	Cat. No	Order
TargetSeq® Human HLA Panel	T216V1	16 rxn	PT1001831	Info
TargetSeq® Human HLA Panel	T216V1	96 rxn	PT1001832	Info
TargetSeq® Human MHC Panel	T231V2	16 rxn	PT1002021	Info
TargetSeq® Human MHC Panel	T231V2	96 rxn	PT1002022	Info

Resources

Demo DataSet

TargetSeq® MHC Panel (T231V2) demo data
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TargetSeq® HLA Panel (T216V1) demo data
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bed File

TargetSeq® MHC Panel (T231V2) bed file
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TargetSeq® HLA Panel (T216V1) bed file
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Articles

Capturing 3D Chromatin Maps of Human Primary Monocytes: Insights From High-Resolution Hi-C

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Yu Xia; Xiaowen Liu; Wenli Mu; Chunyan Ma; Laicheng Wang; Yulian Jiao; Bin Cui; Shengnan Hu; Ying Gao; Tao Liu; Huanxin Sun; Shuai Zong; Xin Liu; Yueran Zhao

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Frontiers in Immunology 2022;13:837336 DOI:10.3389/fimmu.2022.837336

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Gene	Cap1	Cap2	Cap3
A	A02:06:01;A31:01:02	A02:06:01;A31:01:02	A02:06:01;A31:01:02
B	B40:02:01;B46:01:01	B46:01:01;B40:02:01	B40:02:01;B46:01:01
C	C01:02:01;C03:04:01	C01:02:01;C03:04:01	C01:02:01;C03:04:01
DRB1	DRB104:07:01;DRB114:03:01	DRB104:07:01;DRB114:03:01	DRB114:03:01;DRB104:07:01
DRB3	DRB301:01:02;DRB301:01:02	DRB301:01:02;DRB301:01:02	DRB301:01:02;DRB301:01:02
DRB4	DRB401:03:01;DRB401:03:01	DRB401:03:01;DRB401:03:01	DRB401:03:01;DRB401:03:01
DQB1	DQB103:01:01;DQB103:02:01	DQB103:01:01;DQB103:02:01	DQB103:01:01;DQB103:02:01
DQA1	DQA105:03:01;DQA103:01:01	DQA105:03:01;DQA103:01:01	DQA105:03:01;DQA103:01:01
DPB1	DPB105:01:01;DPB102:01:02	DPB105:01:01;DPB102:01:02	DPB105:01:01;DPB102:01:02
DPA1	DPA101:03:01;DPA102:02:02	DPA102:02:02;DPA101:03:01	DPA101:03:01;DPA102:02:02
DOA	DOA01:01:02;DOA01:01:01	DOA01:01:02;DOA01:01:01	DOA01:01:02;DOA01:01:01
DOB	DOB01:01:05;DOB01:04:01	DOB01:04:01;DOB01:01:05	DOB01:04:01;DOB01:01:05
DMA	DMA01:01:01;DMA01:01:01	DMA01:01:01;DMA01:01:01	DMA01:01:01;DMA01:01:01
DMB	DMB01:03:01;DMB01:02:01	DMB01:03:01;DMB01:02:01	DMB01:02:01;DMB01:03:01