TargetSeq® Human HLA Panel and TargetSeq® Human MHC Panel are HLA gene detection products based on TargetSeq® hybridization capture technology developed by iGeneTech. These panels are highly efficient in capturing the polymorphic region and combined with NGS technology to achieve high-precision typing of the HLA gene at 6 sites, and can be widely used in the field of immunology research.
TargetSeq® Human HLA Panel is designed to detect 6 genes related to human leukocyte antigens (HLA) including the full-length sequences of HLA class I molecules A, B, and C genes and the full exon sequences of HLA class II molecules DRB1, DQB1, and DPB1 genes. Based on typing analysis, this panel can be applied to transplant mapping, immune disease research, drug allergy, and other directions.
TargetSeq® Human MHC Panel is designed for the full length of the human leukocyte antigen (HLA) gene and contains the traditional HLA region (~3.37 Mb) and its flanking region (~1.6 Mb). A total of 8 haplotypes, which are PGF, COX, QBL, APD, DBB, MANN, MCF, and SSTO, are also included to achieve high coverage of HLA polymorphic regions. It can be used for the study of immune diseases and oncology research, or can provide reference for tissue and organ transplantation through data analysis, etc.
Panel Number | TargetSeq® HLA Panel：T216V1
TargetSeq® MHC Panel：T231V2 |
Technical Platform | TargetSeq® Hybridization Capture Sequencing |
Coverage Size | HLA Panel：15.6 kb
MHC Panel：4.9 Mb |
Reference Database | IMGT/HLA |
Reference Genome | GRCh37 (hg19) |
Coverage | •HLA Panel: Full length of HLA I-A, -B, -C genes and exons of HLA II-DRB1, -DQB1, -DPB1 genes
•MHC Panel: HLA regions and their flank regions, 8 haplotypes of PGF, COX, QBL, APD, DBB, MANN, MCF and SSTO |
Storage | <-70℃ |
Sequence Platform | Illumina / MGI |
Recommend Sequencing Read Length | PE150 |
Recommend Sequencing Data Size and Depth | HLA Panel：0.2 Gb / 1,000X
MHC Panel：10 Gb |
High resolution, enabling 6 sites of high-precision genotyping.
Rigorous design, with probes achieving high coverage of the target region.
Flexible custom, with the possibility of customizing customer-specific HLA typing products based on actual needs.
Nearly 100 samples were used for library construction by IGT® Enzyme Plus Library Prep Kit, and TargetSeq® Human HLA Panel was for target enrichment. After sequencing with PE150, HLA-HD analysis software was used for typing. The results showed up to 100% consistancy with the HLA typing results of the conventional PCR SBT method, allowing high-precision genotyping at 6 sites. The figure on the right shows an example of HLA 6 sites high-precision typing results.
| Gene | HLA Typing Result |
| HLA-A | A*02:01:01; A*01:01:01 |
| HLA-B | B*08:01:01; B*35:32:02 |
| HLA-C | C*07:01:01; C*04:01:01 |
| HLA-DQB1 | DQB1*02:01:01G; DQB1*05:01:01 |
| HLA-DPB1 | DPB1*04:01:01; DPB1*03:01:01 |
| HLA-DRB1 | DRB1*03:01:01G; DRB1*07:01:01 |
Libraries of 2 gDNA samples were constructed by IGT® Enzyme Plus Library Prep Kit, and TargetSeq® Human MHC Panel was used for target enrichment. After sequencing with PE150, the results showed that at 200X average sequencing depth, the coverage was >97%, with homogeneity (T20%X coverage rate) >90% and capture efficiency >77%, showing that it could achieve high coverage and effective enrichment of MHC region.
| Sample Type | Coverage rate(%) | Target Reads capture rate(%) | Target Mean depth | T 20X coverage rate(%) | T 20%X coverage rate(%) |
| gDNA | 97.27 | 77.27 | 189.71 | 91.80 | 90.38 |
| gDNA | 97.31 | 78.01 | 166.05 | 91.43 | 90.50 |
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