Name: HRD Panel & HRR Panel
Brand: iGeneTech Bioscience Co., Ltd.

Overview

TargetSeq® HRD Panel covers 37,000 SNPs to calculate LOH (genomic heterozygous deletion), TAI (telomere allele imbalance), and LST (large fragment migration) for the HRD score, while TargetSeq® HRR Panel is used for mutation detection of 39 core genes in HRR pathway.

TargetSeq® HRD & HRR Panel v2.0 is a combined panel used for the HRD score assessment as well as mutation detection of 39 core genes in HRR pathway in a single panel, which can be used for risk prediction, medication guidance, molecular typing, and prognosis monitoring.

Product Parameter

Panel Number	TargetSeq® HRD Panel：T974V1hg38 TargetSeq® HRR Panel：T975V1hg38 TargetSeq® HRR & HRD Panel v2.0：T976V1hg38
Technical Platform	TargetSeq® Hybridization Capture Sequencing
Coverage Size	TargetSeq® HRD Panel：3.9 Mb TargetSeq® HRR Panel：177.9 kb TargetSeq® HRR & HRD Panel v2.0：4.1 Mb
Reference Database	dbSNP、1000Genomes
Reference Genome	GRCh38 (hg38)
Coverage	•TargetSeq® HRD Panel: 37,000 HRD-related SNPs •TargetSeq® HRR Panel: Exons and splicing regions of 39 core HRR genes •TargetSeq® HRD & HRR Panel V2: 37,000 HRD-related SNPs and Exons and splicing regions of 39 core HRR genes
Storage	-20℃±5℃
Sequence Platform	Illumina / MGI
Recommend Sequencing Read Length	PE150
Recommend Sequencing Data Size and Depth	TargetSeq® HRD Panel：6 Gb / 500X TargetSeq® HRR Panel：0.5Gb / 1,000X TargetSeq® HRR & HRD Panel v2.0：3Gb / HRD 200×, HRR 500X

Advantages

Well curated SNPs and genes for TargetSeq® HRD Panel and TargetSeq® HRR Panel based on relevant databases.
TargetSeq® HRR Panel designed and screened 39 core genes most associated with the HRR pathway based on the latest NCCN guidelines, oncology drug clinical trials, and top scientific research results.
Optimized probe amount to achieve the final HRD: HRR depth ratio of 1:4 in TargetSeq® HDR & HRR Panel v2.0, ensuring effective detection with lower sequencing cost.
Support semi-customization or used as a spike-in module to other panels.

Performance

Excellent Performance

The sequencing results of 3 gDNA standard samples by using TargetSeq® HRD & HRR Panel v2.0 showed excellent performance in coverage rate, capture rate and uniformity.

Table 1. Data QC of standard reference by using TargetSeq® HRD Panel and TargetSeq® HRR Panel

Panel	Sample	Raw bases(Mb)	Target mean depth	Target reads mapping rate(%)	Target reads capture rate(%)	T 20%X coverage rate（%）
TargetSeq® HRD Panel	Sample1	4043.32	375.13	98.9	81.93	95.11
TargetSeq® HRR Panel	Sample2	279.67	650.55	99.49	71.82	98.31
TargetSeq® HRR & HRD Panel V2.0	Sample3	4268.73	919.84	99.27	71.24	92.58

Consistent Trends in HRD Score and HRR Mutations

By testing 6 tumor samples with both HRD and HRR panel, the HRR genes mutation status showed consist trends with the calculated HRD score, indicating high co-relation and accuracy.

Table 2. Data from different tumor tissue samples by using TargetSeq® HRD Panel and TargetSeq® HRR Panel

Sample	LOH	LST	TAI	HRD-score	HRR Mutation
Sample1	8	21	12	41	Germline variants of uncertain significance
Sample2	3	2	3	8	Somatic or Germline variants of uncertain significance
Sample3	9	14	25	48	Somatic likely pathogenic variants
Sample4	24	31	27	82	Somatic or Germline pathogenic variants
Sample5	4	10	7	21	Germline variants of uncertain significance
Sample6	33	32	27	92	Somatic pathogenic variants

Product Name

Panel Number

Set

Cat. No

TargetSeq® HRD Panel

T974V1hg38

16 rxn

PH2000971

96 rxn

PH2000972

TargetSeq® HRR Panel

T975V1hg38

16 rxn

PH2003711

96 rxn

PH2003712

TargetSeq® HRR & HRD Panel v2.0

T976V1hg38

16 rxn

PH2003801

96 rxn

PH2003802

Resources

Demo DataSet

TargetSeq® HRR Panel (T975V1hg38) demo data

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TargetSeq® HRD Panel (T974V1hg38) demo data

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TargetSeq® HRD & HRR Panel v2 (T976V1hg38) demo data

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bed File

TargetSeq® HRR Panel (T975V1hg38) bed file

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TargetSeq® HRD Panel (T974V1hg38) bed file

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TargetSeq® HRD & HRR Panel v2 (T976V1hg38) bed file

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HRD Panel & HRR Panel

Overview

Product Parameter

Advantages

Performance

Ordering Info

Resources