Characterized by maternal inheritance, multiple copies, high heterogeneity, and high mutation rates, human mitochondria are important in human health as well as molecular anthropology. iGeneTech Bioscience offers 2 full length human mitochondria genome capture solutions with hybridization capture and multiplex amplicon technologies, where the hybridization capture one can also be used as spike-in module in hybridization capture panel customization.
Panel Number | T033 (Hybridization Capture)
A102V1 (Multiplex Amplicon) |
Technical Platform | T033: TargetSeq® Hybridization Capture Sequencing
A102V1: MultipSeq® Multiplex Amplicon Sequencing |
Coverage Size | 16.5 kb |
Reference Database | RefSeq |
Reference Genome | GRCh37 (hg19) |
Coverage | Full length of human mitochondria |
Storage | T033：<-70℃
A102V1：-20℃±5℃ |
Sequence Platform | T033：Illumina / MGI
A102V1：Illumina |
Recommend Sequencing Read Length | PE150 |
Recommend Sequencing Data Size and Depth | 0.2 Gb / 5000X |
13 Protein-coding Genes | MT-ND1、MT-ND2、MT-ND3、MT-ND4、MT-ND4L、MT-ND5、MT-ND6、MT-CYB、MT-CO1、MT-CO2、MT-CO3、MT-ATP6、MT-ATP8 |
22 tRNA-coding Genes | MT-TA、MT-TR、MT-TN、MT-TD、MT-TC、MT-TE、MT-TQ、MT-TG、MT-TH、MT-TI、MT-TL1、MT-TL2、MT-TK、MT-TM、MT-TF、MT-TP、MT-TS1、MT-TS2、MT-TT、MT-TW、MT-TY、MT-TV |
2 rRNA-coding Genes | MT-RNR1、MT-RNR2 |
1 Regulatory Region | D-loop Region |
| Hybrid Capture Solution | Multiplex Amplicon Solution | |
Technology | TargetSeq® Hybridization Capture Sequencing Technology | MultipSeq® Multiplex Amplicon Sequencing Technology |
Product | TargetSeq® Human Mitochondrial Panel | MultipSeq® Human Mitochondrial Research Assay (for Illumina) |
Platform | Illumina & MGI | Illumina |
Advantage | Can be used as spike-in module | Fast, easy and cost-efficiency |
Both solutions can achieve excellent data performance to ensure the full-length capture of mtDNA.
| Product Name | Panel Number | Set | Cat. No |
| TargetSeq® Human Mitochrondrial Panel | T033 | 16 rxn | PT1000021 |
| 96 rxn | PT1000022 | ||
| MultipSeq® Human Mitochrondrial Research Assay (for Illumina) | A102V1 | 16 rxn | M62101 |
| 96 rxn | M62102 | ||
| 960 rxn | M62103 |
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