Overview

IGT® UMI Adapter & UDI Primers is a series of kits used to detect samples with low-frequency mutations. Unique molecular identifiers (UMIs) are a type of molecular barcoding that tag each molecule in the library, which can reduce the false-positive rate and increase the sensitivity of mutation detection. iGeneTech offers 64 different 6-bp UMI adapters with edit distance not less than 3, which is enough to distinguish between original molecules and correct the errors during sequencing.

iGeneTech provides 384 combinations of different UDI primers for high-throughput sequencing in a single lane that reduces the index contaminations due to index hopping.

iGeneTech also offers Adapter & Adapter & UDI Primers without UMI technology.

Advantages

High accuracy: 99.9% background noise filtered out by UMI correction
High sensitivity of detection: sensitivity and stable mutation detection of cfDNA samples with mutation rates as low as 0.02%
Reliable UMIs: Special non-random UMI design strategy with edit distance not less than 3
Low index cross-contamination rate: Unique Dual Index (UDI) primers included to filter the hopped reads.

Technical Principle

Illumina

MGI

Principle for data correction UMI adapter

Performance

High Accuracy and Sensitivity of Low-Frequency Mutation Detection

Test of low-frequency Horizon 0.1% cfDNA reference standard using IGT® UMI Adapter & UDI Primers successfully detected all 8 mutations with reliable mutation rate.

EGFR L858R	EGFR T790M	EGFR delE746-A750	EGFR V769-D779insASV	KRAS G12D	NRAS Q61K	NRAS Q61K	NRAS A59T	PIK3CA E545K
0.08%	0.10%	0.09%	0.06%	0.10%	0.10%	0.12%	0.16%	0.21%

Significantly Reduce the False-Positive Rate

UMI can significantly reduce the false-positive rate by error correction. The following figure shows the raw data and corrected data with standard, SSCS and DSC de-duplication correction.

The DSC correction using UMI reduced the number of mutations with VAF less than 3% from 526 to 15, including all the 8 known mutations.

Ordering Info

UMI Adapter & UDI Primers

Product Name	Set	Cat. No
IGT® UMI Adapter & UDI Primer1-96/ 97-192/193-288/289-384 (for Illumina, plate)	96*1 rxn	C10092 / C10102 / C10112 / C10122
IGT® UMI Adapter & UDI Primer 1-16 (for Illumina, tube)	16*1 rxn	C10131
IGT® UMI Adapter & UDI Primer1-96/ 97-192/193-288/289-384 (for Illumina, tube)	96*10 rxn	C10093 / C10103 / C10113 / C10123
IGT® UMI Adapter & UDI Primer 1-96/ 97-192/193-288/289-384 (for MGI, plate)	96*1 rxn	C10232 / C10242 / C10252 / C10262
IGT® UMI Adapter & UDI Primer 1-16 (for MGI, tube)	16*1 rxn	C10271
IGT® UMI Adapter & UDI Primer 1-96/ 97-192/193-288/289-384 (for MGI, tube)	96*10 rxn	C10233 / C10243 / C10253 / C10263

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