TargetSeq® Universal Blocking Oligo series products are used to block self-ligation of adapters to reduce non-specific adapter interaction during probe hybridization and thus to increase data utilization. iGeneTech offers 2 versions of universal blocking oligo for different library input in hybridization workflow, i.e., TargetSeq® Universal Blocking Oligo and TargetSeq® Eco Universal Blocking Oligo for up to 6 μg and 3 μg libraries, respectively.
Suitable for various types of libraries
Stable and excellent performance with different panels and inputs
Pre-mixed reagents for simple operation.
TargetSeq® Universal Blocking Oligo series products are suitable for different library types. The following figures are test results for single- and dual-indexed libraries with or without UMI, and the results showed that the performance are stable and excellent in all tested library types.
TargetSeq® Universal Blocking Oligo series products work well with panels of different region sizes. The results are shown in the figures below for small, median and large panels of region size of 70 kb, 1 Mb and 34 Mb, respectively.
TargetSeq® Universal Blocking Oligo series products are stable for different library input amounts. In a test with different input library amount of 750 ng, 3 μg and 6 μg, the results were stable among all samples.
TargetSeq® Universal Blocking Oligo series products performed stable and well in multiplexing testing with 1-Plex (500 ng), 6-Plex (3 μg) and 12-Plex (6 μg).
| Product Name | Set | Cat. No |
| TargetSeq® Universal Blocking Oligo (for Illumina) | 4 rxn | C80490 |
| 16 rxn | C80491 | |
| 96 rxn | C80492 | |
| TargetSeq® Universal Blocking Oligo (for MGI DI) | 4 rxn | C80520 |
| 16 rxn | C80521 | |
| 96 rxn | C80522 | |
| TargetSeq® Eco Universal Blocking Oligo (for Illumina) | 16 rxn | C80501 |
| 96 rxn | C80502 | |
| TargetSeq® Eco Universal Blocking Oligo (for Illumina Nextera) | 16 rxn | C80511 |
| 96 rxn | C80512 | |
| TargetSeq® Eco Universal Blocking Oligo (for MGI SI) | 16 rxn | C80541 |
| 96 rxn | C80542 | |
| TargetSeq® Eco Universal Blocking Oligo (for MGI DI) | 16 rxn | C80531 |
| 96 rxn | C80532 | |
| TargetSeq® Eco Universal Blocking Oligo (for Illumina ssDNA Library) | 16 rxn | C80791 |
| 96 rxn | C80792 |
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